ABSTRACT
Objective To establish a method for the determination of aloin of compound aloe ointment. Methods HPLC method was used with Agilent Zorbax-C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-0.08%Sodium 1-heptanesulfonate (22∶78). The flow rate was 1.0 ml/min,the column temperature was 30 ℃, and the UV detector was set at 355 nm. Results The linear ranges of aloin was 0.037~0.74 mg/ml(r=0.999 8). The average recovery of aloin was 98.7%. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of aloin in compound aloe ointment.
ABSTRACT
<p><b>BACKGROUND</b>Gallbladder carcinoma (GBC) has a high mortality rate, requiring synergistic anti-tumor management for effective treatment. The myxoma virus (MYXV) exhibits a modest clinical value through its oncolytic potential and narrow host tropism.</p><p><b>METHODS</b>We performed viral replication assays, cell viability assays, migration assays, and xenograft tumor models to demonstrate that bone marrow-derived stem cells (BMSCs) may enhance efficiency of intravenous MYXV delivery.</p><p><b>RESULTS</b>We examined the permissiveness of various GBC cell lines towards MYXV infection and found two supported single and multiple rounds of MYXV replication, leading to an oncolytic effect. Furthermore, we found that BMSCs exhibited tropism for GBC cells within a Matrigel migration system. BMSCs failed to affect the growth of GBC cells, in terms of tumor volume and survival time. Finally, we demonstrated in vivo that intravenous injection of MYXV-infected BMSCs significantly improves the oncolytic effect of MYXV alone, almost to the same extent as intratumoral injection of MYXV.</p><p><b>CONCLUSION</b>This study indicates that BMSCs are a promising novel vehicle for MYXV to clinically address gallbladder tumors.</p>
Subject(s)
Animals , Female , Humans , Mice , Bone Marrow Cells , Cell Biology , Cell Movement , Physiology , Cell Survival , Physiology , Gallbladder Neoplasms , Therapeutics , Virology , Immunohistochemistry , Myxoma virus , Virulence , Stem Cells , Cell Biology , Physiology , Virus Replication , Physiology , Xenograft Model Antitumor AssaysABSTRACT
Objective To establish a HPLC method for the determination of berberine hydrochloride in Jiawei Ermiao Wan.Methods The RP-HPLC system consisted of the Luna-C18 (4.6 mm × 250 mm,5 μm) column,the mobile phase consisted of acetonitrile-0.1％H3PO4(45:55),the flow rate was 1.0mL · min-1,the column temperature was 30℃,The UV detector was set at 245nm.Results The linear response range was 0.071～1.42 mg· ml-1 (r=0.9998).The average recovery of mangiferin was 99.2％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of berberine hydrochloride in Jiawei Ermiao Wan.
ABSTRACT
Objective To observe the efficacy of percutaneous hepatic cryoablation for the treatment of primary or meta-static hepatic malignancies (<5 cm in diameter).Methods A total of 31 patients (39 tumors <5 cm in diameter) were treated with argon-helium cryoablation system under the guidance of CT or ultrasound.Results Tumor ablation range was 90%-100% in 39 lesions,including 69.23% (27/39) complete ablation.The 1- and 2-year survival rate was 90.32% (28/31) and 61.29% (19/31),respectively.No bleeding and injury of blood vessel or bile duct was noted.Complications of cryoablation included intraoperative shivering in 4 (12.90%) patients,postoperative fever (37.12-38.25℃ in 7 (22.58%) patients and hepatic pain in 6 (19.36%) patients.One patient had severe pain relief until 2 h after cryosurgery with ice-cold skin temperature and stable life index,analgesic had little effect,and no bleeding was found on CT image.Other patients had slight or moderate pain and remained untreated.Conclusion Percutaneous targeted argon-helium cryoablation is a feasible and safe technique in the treatment of small primary or metastatic hepatic malignancies not suitable for resection.